Study on the Comparison of Three Typical Gas Flow Standards for Calibrating Low Gas Flowmeter

A gas flowmeter for measuring low flow rate has been widely used in the field of medical, health, environmental protection, energy industry, aerospace, etc. To against Covid-2019, the requirement on the low flow rate has been increasing dramatically. At present, the typical standard devices for calibrating low gas flowmeter mainly include standard bell provers of gas flow, standard piston provers of low gas flow and standard laminar of low gas flow. Different measuring principles are adopted among these typical standard devices. To ensure the consistency of these typical standard devices, a comparison test is performed. The standard devices used in the comparison are of the same accuracy grade, with an extended uncertainty of 0.2%(k=2). The piston-type gas flow calibrator of grade 1.0 is selected as the transfer standard, and three flow points with high flow rate, medium flow rate and low flow rate are selected for test. The consistency of measurement results is evaluated by normalized deviation En. The comparison results are acceptable which show that three typical standard devices are accurate and reliable. © FLOMEKO 2022.All rights reserved

Fast proteomics with dia-PASEF and analytical flow-rate chromatography.

Increased throughput in proteomic experiments can improve accessibility of proteomic platforms, reduce costs, and facilitate new approaches in systems biology and biomedical research. Here we propose combination of analytical flow rate chromatography with ion mobility separation of peptide ions, data-independent acquisition, and data analysis with the DIA-NN software suite, to achieve high-quality proteomic experiments from limited sample amounts, at a throughput of up to 400 samples per day. For instance, when benchmarking our workflow using a 500-µL/min flow rate and 3-min chromatographic gradients, we report the quantification of 5211 proteins from 2 µg of a mammalian cell-line standard at high quantitative accuracy and precision. We further used this platform to analyze blood plasma samples from a cohort of COVID-19 inpatients, using a 3-min chromatographic gradient and alternating column regeneration on a dual pump system. The method delivered a comprehensive view of the COVID-19 plasma proteome, allowing classification of the patients according to disease severity and revealing plasma biomarker candidates.

Carbon Dioxide Monitoring in Refuse Collection Vehicle Cabins to Reduce the Risk of SARS-CoV-2 Airborne Transmission

During the COVID-19 pandemic, essential workers such as waste collection crews continued to provide services in the UK, but due to their small size, maintaining social distancing inside waste collection vehicle cabins is impossible. Ventilation in cabins of 11 vehicles operating in London was assessed by measuring air supply flow rates and carbon dioxide (CO2) in the driver's cabin, a proxy for exhaled breath. The indoor CO2 indicated that air quality in the cabins was mostly good throughout a working day. However, short episodes of high CO2 levels above 1500 ppm did occur, mainly at the beginning of a shift when driving towards the start of their collection routes. This data indicated that the ventilation systems on the vehicles were primarily recirculating air and the fresh air supply made up only 10-20 % of the total airflow. Following recommendations to partly open windows during shifts and to maintain ventilation systems, a second monitoring campaign was carried out, finding on average, an improvement in ventilation on board the vehicles. © 2022 17th International Conference on Indoor Air Quality and Climate, INDOOR AIR 2022. All rights reserved.

Post-Occupancy study of indoor air quality in university laboratories during the pandemic

This post-occupancy study aims to assess the indoor air quality (IAQ) and ventilation performance in workshops and laboratories of a UK university during the COVID-19 pandemic. Supply airflow rates and CO2 were monitored as a proxy for evaluating ventilation performance. Additionally, particulate matter (PM10) was monitored to address the occupant's concerns about dust. Monitoring showed that maximum CO2 values recorded are mostly below 1000 ppm, with weekly averages below 520 ppm. This was expected as the supply airflow rates were significantly larger than recommended 10 l/s per occupant. Despite the large flow rates, PM10 levels in some laboratories were above the threshold value of 50 [μg/m3] supporting the poor IAQ claims of the occupants. The study indicated the room air re-circulation and indoor activities as the likely reasons for the elevated PM10 levels and some practical operational solutions were suggested for IAQ concerns. © 2022 17th International Conference on Indoor Air Quality and Climate, INDOOR AIR 2022. All rights reserved.

Occupational Health Hazard Identification and Risk Assessment with Introduction of Biomass in Coal Based Captive Power Plant

Introduction: Occupational Health should aim at the Promotion and Maintenance of the highest degree of physical, mental, and social well-being of all the employees. A pilot project was taken up due to acute shortages of coal during the COVID Pandemic, on industrial level, mixing of biomass with coal at a ratio of 20:80 respectively was considered as a good raw material. With introduction of biomass, workers were exposed to different organic substances either directly through dermal route or respirable dust with risk of becoming victims to Occupational diseases. Objective(s): The objective of the study is to identify and mitigate occupational health hazard of various nature prevailing at workplace after introduction of new raw materials;to safeguard the workforce from discomfort and occupational illness and to provide healthy working environment at RIL-Hazira. Method(s): Walk through survey was initiated by team of industrial hygienist and medical officer along with the process engineer. Subsequent workplace evaluation was done according to ACGIH screening criteria for respirable dust & VOC monitoring. To measure airborne respirable contaminants, we have considered housekeeping staff, operator, field executive, Boiler operation engineer which were found more likely to be at the risk of airborne contaminant exposure. To identify the concentration of contaminants, personal air sampler (SKC Make) was used for collection of respirable dust samples for different job category of workers. NIOSH 600 method was used for exposure assessment and samples were collected by using PVC filter used at the flow rate of 2.5 lpm. The composition of biomass pellets was received from biomass team & chemical analysis of biomass was done at our laboratory. Occupational Diseases known to be caused by organic agricultural compounds used as fuel were taken into account such as Bagasossis, farmer's lung & other hypersensitivity pneumonias, non-tubercular mycobacterial infections, infections caused by various fungi & bacteria. Prevention & Control measures were taken during the project such as modification of process, local exhaust ventilation, worker education on different diseases, personal hygiene, use of PPE, good housekeeping. Result(s): Through effective Risk assessment, Hazard Identification and measures taken to mitigate Occupational health hazards, no occupational health disease was reported after implementation of the change in process in a total of 55 identified workers. Moving forward these workers will be periodically monitored. The amount of total respirable dust was reduced by approx. 10- 25% at different location of the plant after control measures taken. This project also brought huge monetary benefits to the plant. Leading forward as the pilot project for introduction of biomass was a great success it has been planned to be scaled up to 40% mixture of biomass.

An Rp-Hplc Method for the Quantitative Determination of Remdesivir in Intravenous Dosage Form

The worldwide epidemic of Coronavirus disease 2019 (COVID-19), caused by a new virus known as severe acute respiratory syndrome (SARS) coronavirus 2, has posed a growing threat to public health (SARS-CoV-2). The only antiviral drug authorized by the FDA for treating adult and pediatric patients hospitalized with a severe disease is remdesivir, which is given intravenously (IV). Although only a few methods for estimating remdesivir in pharmaceutical formulations using high-pressure liquid chromatography (HPLC) have been described, its determination still requires an accurate, precise, quick, and easy analytical methodology. The main goal of this study was to develop and validate a reliable and accurate HPLC method for quantitative estimation of remdesivir in its intravenous dosage formulation. The separation was performed on a C18 (4.6 mm x 150 mm, 5.0 microm) column with a flow rate of 0.7 mL/min and a total run duration of 6 minutes using a simple isocratic mobile phase of acetonitrile and 0.1 percent formic acid. The method was validated for the system suitability, linearity, precision, accuracy, robustness, and others as per the International Council for Harmonization (ICH) Q2 (R1) guideline. The results show that the method for measuring remdesivir using HPLC is simple, quick, sensitive, accurate, precise and robust. The described approach was successfully used to quantify remdesivir in a commercially available pharmaceutical formulation.Copyright All © 2023 are reserved by International Journal of Pharmaceutical Sciences and Research.

Study of Air Recirculation Zones in Shared Spaces

The Air borne transmission is a very big concern for highly infectious diseases like Covid-19 and other airborne diseases. A micro droplet and aerosol can be carried out in the air and can remain flowing in air over a distance in a confined space, leading to affecting high number of people getting prone to infection and it is very dangerous in enclosed spaces or shared spaces. Public places, shared facilities are the areas, where infectious aerosol can be present in the air for a long duration. Ventilation of closed spaces, shared spaces is the need of hour to have analysed and deep study in context of infectious airborne diseases. Introduction of fresh air into the enclosed environment at regular interval of times may lead to fast dilution of air present in the enclosed space. The prominent building codes and HVAC guidelines allows as to calculate ACPH (Air changes per hour) in an enclosed space as per the occupancy and flow rate. The age of air is the criteria to define the amount of air residing in the enclosed space when it enters the space till its exhaust from that space. The more the age of air in the particular area the more can be the infection probability among the occupants. It is predominant to study the airflow pattern caused due to ventilation which can be collaborated with age of air to know about the infection probability. Typically, a classroom geometry is assumed with inlet outlet boundary conditions where exhaust fan is playing a major role of displacement ventilation. Study of air recirculation zones and dead zones is the point of interest of this study. Computational fluid dynamics is the most powerful tool in the present era to study the air flow pattern in enclosed and shared spaces.Copyright © 2022, Anka Publishers. All rights reserved.

A Smart CO2-Based Ventilation Control Framework to Minimize the Infection Risk of COVID-19 In Public Buildings

This study aims to present a smart ventilation control framework to reduce the infection risk of COVID-19 in indoor spaces of public buildings. To achieve this goal, an artificial neural network (ANN) was trained based on the results from a parametric computational fluid dynamics (CFD) simulation to predict the COVID-19 infection risk according to the zone carbon dioxide (CO2) concentration and other information (e.g., zone dimension). Four sample cases were analyzed to reveal how the CO2 concentration setpoint was varied for a given risk level under different scenarios. A framework of smart ventilation control was briefly discussed based on the ANN model. This framework could automatically adjust the system outdoor airflow rate and variable air volume (VAV) terminal box supply airflow rate to meet the needs of reducing infection risk and achieving a good energy performance. © International Building Performance Simulation Association, 2022

Can we predict HoLEP surgery operating times for a given prostate volume to enhance theatre utilization?

Introduction & Objectives: Holmium laser enucleation of the prostate (HoLEP) has the strongest evidence base for bladder outlet surgery, despite its steep learning curve. Rapid enucleation rates can be achieved in established hands with day-case surgery being the norm in service delivery. We have previously shown the validity of such a model. With the post Covid surgical backlog we have developed a tool to support theatre utilization based on established surgeon specific operating room (OR) times for a given prostate volume in our unit based on almost 1100 cases. Material(s) and Method(s): Four HoLEP naive surgeons completed 1096 HoLEPs over 7.5 years using a 50 Watt (W) Holmium laser (Auriga XL, Boston Scientific Inc., Piranha morcellator, Richard Wolf). Pre and post-operative data including TRUS/MRI volume, flow rate, residual volume, international prostate symptom score, quality of life, stop-clock enucleation, morcellation and total operating room (OR) times, hospital stay, histology, haemoglobin, creatinine, sodium and catheter times were prospectively recorded. Mentorship was provided by a senior 100W HoLEP surgeon from an adjoining hospital. Result(s): The data was independently analysed by a bio-statistician (IN). Statistical regression analysis of unit and surgeon specific OR times vs prostate volume were used to produce predictive linear graphs of OR times (mins) for a given prostate volume for individual surgeons and the unit. [Figure presented] Conclusion(s): Use of surgeon-specific and unit specific OR times allows the opportunity to maximize theatre operating schedules to help tackle the post Covid surgical backlog. We encourage this process for index specialist procedures across units.Copyright © 2023 European Association of Urology. Published by Elsevier B.V. All rights reserved.

Effect of energy renovation on indoor air quality and thermal environment in winter of a primary school in a highly polluted French alpine valley

With increasing the COVID-19 pandemic and the time spent indoor, there is a growing research interest in the issue of Indoor Environmental Quality in building including thermal environment and indoor air quality (IAQ). Research and intervention in schools are a particular focus, as children are especially vulnerable to air pollution. The aim of this study is to assess the impact of energy renovation, including the installation of a balanced ventilation system with a filter (F7 type), on the IAQ of a school building located in a polluted outdoor environment. The study is based on measurements of some parameters of thermal environment and IAQ. To this end, two classrooms were chosen for two measurement campaigns. Each campaign covered 2 months in winter in 2018 and 2020 before and after renovation, respectively. The measurements included ventilation airflow rates, temperature, relative humidity, carbon dioxide, and particle concentration (PM2.5). The main result of installing the balanced ventilation was an increase in the air change rate from 0.1 h−1 and 0.05 h−1 before the renovation to 1.5 h−1 and 1.7 h−1 after the renovation, for classroom 1 and classroom 2, respectively. This increase changed the ICONE air stuffiness level from average air stuffiness to fresh air (no air stuffiness). However, this increase resulted in a significant entry of outdoor particles. As consequence, the highest indoor/outdoor concentration ratio (57%) was observed after the renovation. All these results highlights that ventilation performance should be extended to parameters as filtration efficiencies in order to increase IAQ. © 2023 Elsevier Ltd

Computational fluid dynamic analysis of corona virus patients breathing in an airplane

Effective ventilation systems are essential to control the transmission of airborne aerosol particles, such as the SARS-CoV-2 virus in aircraft cabins, which is a significant concern for people commuting by airplane. Validated computational fluid dynamic models are frequently and effectively used to investigate air distribution and pollutant transport. In this study, the effectiveness of different ventilation systems with varying outlet vent locations were computationally compared to determine the best ventilation system for minimizing the risk of airborne transmission. The cabin air conditioning system was optimized to determine how design variables (air inlet temperature, outlet valve width and location, and mass flow rate) affect output parameters, including particle residence time, age of air, and thermal comfort conditions. Inlet mass flow rate was observed to be an influential variable impacting all output parameters, especially on age of air, where it was the most influential. In contrast, the least effective variable was width of the outlet valve, which only affected the particle residence time. Also, Predicted Mean Vote and Predicted Percentage Dissatisfied indices were the most affected by air inlet temperature, which had an inverse relation, while the outlet valve location had the greatest effect on particle residence time. © 2023 Author(s).

Poster Presentation: AB No: 62: Comparison of Functional Capacity and Peak Expiratory Flow Rate in Covid-19 survivors with Healthy Individuals – A Pilot Study

Purpose: To compare functional capacity and peak expiratory flow rate in COVID-19 survivors and healthy individuals.Relevance of study: COVID 19 has shown to affect the lung parenchyma. For physiotherapists, as they deal with maximising functional potential of patients, knowing status of functional capacity and lung function of COVID-19 survivors may help in understanding patients' problem more effectively and also in making appropriate clinical decisions. Participants: Fifty participants from community of Ahmedabad, males and females of age group 50–65 years, with (group A) & without (group B) history of COVID-19 in the past 12 months, will be included in the study by convenience sampling. Patients with severe musculoskeletal, neurological & cardiac problems, recent injury, fever, cough & cold are excluded. Method: Observational analytical study was conducted. 6minute walk distance for functional capacity and peak expiratory flow rate with a peak flow meter, was obtained. Analysis: Data analysis for 14 participants was done using SPSS version 16 and Microsoft excel 2019. Data was screened for normality using Levene's test. Between group comparison for 6MWD and PEFR was done using Mann-Whitney U test. Level of significance kept at 5%. Results: Mean age in group A was (58.0 + 4.47) years, and in group B was (59.2 + 5.49)years. Difference in mean+SD for PEFR in L/min for group A (414.29 + 186.44) and Group B (344.29 + 66.29) was not significant (U=20, p=0.562). Difference in mean + SD for 6MWD group A (414.76 + 101.99), Group B (481.69 + 90.24) was not significant (U= 13.5, p=0.15). Conclusion: Study is still going. Implications: If the peak expiratory flow rate and functional capacity are reduced post COVID, there will be a need to manage the same.

Impact of COVID 19 infection on cardiopulmonary functions of a cohort of national level athletes in Sri Lanka

Limited evidence exists regarding adverse effects on cardiovascular and pulmonary function in adults affected by covid 19 infection. We describe the cardiopulmonary functions in a cohort of national level athletes with recent covid infection. 42 healthy athletes were evaluated after at least a 10-day period of quarantine after mild covid infection.44% females [n=19] and 56% males [n=23]. The age range was 15 - 38 years. The commonest persistent symptoms were difficulty in breathing (4.8%), cough (7%) and chest tightness (4.8%). PCFS (post covid functional score) was 0 in 93%. The CXR and 6-minute walk test were normal in all. The spirometry was normal in 83% (n=35). The mean FEV1 were 2.68l and 3.75l;mean FVC were 3.09l and 4.6l in females and males respectively. The mid expiratory flow rate (FEF 25-75) was less than the predicted lower limit of normal in 52.4%(mean=2.8L). Cardiac abnormalities were detected in 7%;moderate interventricular septal dyssynchrony, global hypokinesia with mild LV dysfunction and mild pulmonary hypertension. 13 players with persistent difficulty in breathing and chest tightness who had otherwise normal spirometry and echo were referred for CPET. Only 6 players had CPET due to limited resources: evidence of deconditioning (n=3) and cardiac limitation with poor O2 pulse (n=3). 78% (n=33) had normal cardiopulmonary assessment and were referred for graduated return to practice. Abnormalities were identified in 21% (n=9). Mid expiratory flow was reduced in 53% indicating possible effect on peripheral airways post covid. Evaluation of athletes and guidelines on return to practice after covid infection are an important and timely need.

Designing Hybrid Mechanical Ventilator System Based on Arduino and Raspberry Pi 4

Mechanical ventilators are advanced life-supporting machines in this century. The ventilator needs to be safe, flexible, and easy for competent clinicians to use. Since ventilators supply the patient with gas, they need pneumatic components to be present. First technology ventilators were typically powered by pneumatic energy. Gas pressure is used to power ventilators as well as ventilate patients. Nowadays, ventilators are operated electronically with the useful microprocessor tool. This proposal aims to design a simple portable mechanical ventilator that includes measuring some important physiological variables such as respiratory rate, heart rate, and O2 saturation, which can be utilized in hospital and at home. The proposed system includes Arduino, Raspberry pi4, touch screen, and graphical user interface. This study showed a significant individual performance for measuring some important parameters such as flow rate, tidal volume, and minute ventilation. The accuracy of measuring the flow rate was 72%. The Cohen's kappa (CK) was estimated to be 0.61. The accuracy of calculated the tidal volume was estimated at 83% with 0.80 CK. The accuracy of measuring the O2 saturation was estimated at 99% with 0.99 CK. The advantages of the proposed design are cost-effective, safe, flexible, and easy to use. Also, this system is smart and can control its transactions, so it can be used at home without the need for professional help. The operating parameters can also be set by the user with a simple user interface.Copyright © 2022 by ASME.

Recent progress in microfluidic biosensors with different driving forces

Microfluidic biosensors integrating fluid control, target recognition, as well as signal transduction and output, have been widely used in the field of disease diagnosis, drug screening, food safety and environmental monitoring in the past two decades. As the central part and technical characteristics of microfluidic biosensors, the fluid control is not only associated with accuracy and convenience of the sensors, but also affects the material selection and working mode of the sensors. This review summarizes the fluid driving forces for microfluidic biosensors, including gravity, capillary force, centrifugal force, pressure, light, sound, electrical, and magnetic forces. Then, the recent advances in microfluidic biosensors for the detection of viruses, cells, nucleic acids, proteins and small molecules are discussed. Finally, we propose the current challenges and future perspectives of microfluidic biosensors. We hope this review can provide readers with a new perspective to understand the technical characteristics and application potential of microfluidic biosensors.Copyright © 2022 Elsevier B.V.

Ventilation Efficiency According to Tilt Angle to Reduce the Transmission of Infectious Disease in Classroom

Understanding of the droplet transmission of respiratory diseases is necessary to control the outbreak of COVID-19. HVAC systems considering droplet transmission are commonly used to prevent numerous respiratory diseases by reducing indoor virus concentrations. The transmission of the virus was directly related to indoor flow patterns generated by HVAC systems. Thus, a study on operating conditions such as direction or the tilt angle was required. In this study, the effective ventilation rate and probability of droplet transmission according to the tilt angle of supply air and the number of people were studied. A CO2 tracer gas method was used to validate the results of simulations. The breathing plane and personal respiratory zone were introduced for the probability of droplet transmission. The result showed that ventilation performance showed 17% of the maximum difference among tilt angles. Various turbulent kinetic energies were obtained according to the seated positions, resulting in non-uniform CO2 concentration. Numerous conditions were examined with locational analysis of individuals. As a result, the flow rates for ventilation were recommended to be higher than 250 m3/h and 350 m3/h with a tilt angle of 60degree for an occupancy of 8 and 16 people, respectively.Copyright © The Author(s) 2022.

LONG-TERM SUPER HIGH-FLUX HEMODIALYSIS (OR EXPANDED HEMODIALYSIS;HDx) DOES NOT ALTER NUTRITION STATUS WITH THE SIGNIFICANTLY REDUCED MIDDLE-MOLECULE AND PROTEIN-BOUND UREMIC TOXINS IN HEMODIALYSIS PATIENTS

Introduction: Super high-flux hemodialysis (SHF-HD) provides comparable effectiveness in terms of middle-molecule and protein-bound uremic toxin removal to online hemodiafiltration in prevalent end-stage kidney disease (ESKD). However, dialysate albumin loss is raised awareness of the long-term using SHF-HD. The study aims to monitor the long-term use of SHF-HD in nutritional status change and the sustained effect of uremic toxin removal. Method(s): The present study was prospectively conducted on the 15 prevalent ESKD patients from a run-in period of standard high-flux hemodialysis (HF-HD) with ELISIO-H21 dialyzer for 4 weeks to thereafter 15 months follow-up with SHF-HD. The patients provided high-efficiency (high blood flow and dialysate flow rate) SHF-HD using PES17D alpha dialyzers for the first three months. After the amendment protocol, SHF-HD was run with the same type of dialyzer;ELISIO-17Hx (Nipro Corporation, Osaka, Japan) due to the COVID-19 pandemic. Nutritional parameters, BCM;body composition monitor (FMC, Bad Homburg, Germany), and uremic toxins were measured at baseline and every three months during SHF-HD. Result(s): Fourteen of 15 patients could complete the study. One patient was early terminated due to undergoing kidney transplantation. After 15 months of SHF-HD treatment compared to HF-HD at baseline, there was not a statistically significant change in clinical and laboratory parameters on nutritional status. The mean serum albumin levels were 4.09 (1.36) versus 4.01 (0.3) g/dL, respectively (p=0.52), and the mean difference (SE) of normalized protein catabolic rate (nPCR) was -0.04 (0.08), 95% confidence interval [CI] -0.19, 0.11. On the other hand, lean tissue mass (LTM) was significantly decreased, and fat mass was significantly increased (mean difference (SE) of -3.66 (1.07) gram, 95% CI -5.76, -1.55, and 1.79 (0.80), 95% CI 0.21, 3.36). SHF-HD sustainably and significantly removed medium to large middle-molecule uremic toxins including pre-dialysis beta-2 microglobulin, kappa-free light chain, and lambda-free light chain. In addition, protein-bound uremic toxin;indoxyl sulfate was significantly reduced during long-term follow-up using SHF-HD. SHF-HD with PES17D alpha dialyzer resulted in more dialysate albumin leaks than a newer type of SHF-HD with ELISIO-Hx17. Conclusion(s): Long-term use of SHF-HD in ESKD patients was associated with nutritional safety and effectiveness in middle-molecule and protein-bound uremic toxin removal. Although serum albumin and BMI were not changed. LTM was significantly reduced with lower levels of nPCR than in other studies but trended to increase over time. The LTM absolute levels are not below the 10 percentiles of the healthy reference range. Increasing protein intake to reach the current recommendation and physical activity was advised with long-term use of SHF-HD to avoid further reduce LTM. [Formula presented] [Formula presented] [Formula presented] No conflict of interestCopyright © 2023

Tunable MEMS-Based Terahertz Metamaterial for Pressure Sensing Application.

In this study, a tunable terahertz (THz) metamaterial using the micro-electro-mechanical system (MEMS) technique is proposed to demonstrate pressure sensing application. This MEMS-based tunable metamaterial (MTM) structure is composed of gold (Au) split-ring resonators (SRRs) on patterned silicon (Si) substrate with through Si via (TSV). SRR is designed as a cantilever on the TSV structure. When the airflow passes through the TSV from bottom to up and then bends the SRR cantilever, the SRR cantilever will bend upward. The electromagnetic responses of MTM show the tunability and polarization-dependent characteristics by bending the SRR cantilever. The resonances can both be blue-shifted from 0.721 THz to 0.796 THz with a tuning range of 0.075 THz in transverse magnetic (TM) mode and from 0.805 THz to 0.945 THz with a tuning range of 0.140 THz in transverse electric (TE) mode by changing the angle of SRR cantilever from 10° to 45°. These results provide the potential applications and possibilities of MTM design for use in pressure and flow rate sensors.

Stability Indicating RP-HPLC Method for the estimation of Favipiravir in API and Pharmaceutical Dosage Forms (Tablets)

Favipiravir is an anti-viral agent used for the treatment of a large number of RNA viruses including influenza and was considered for the treatment of COVID-19. A new stability indicating RP-HPLC has been developed for the estimation of Favipiravir and the method was validated. Hypersil BDS C18 column was used for the chromatographic study with a flow rate 1ml/min (Detection wavelength 230nm). The LOD and LOQ were found to be 0.2918mug/mL and 0.9207mug/mL respectively. Linearity was observed over the concentration 1-300mug/mL with linear regression equation, y = 28444x + 21853 (R2 = 09998) and the method is precise, accurate and robust. Stress degradation studies were performed and the method was found to be selective and specific. Copyright © RJPT All right reserved u.

Development and Validation of RP-HPLC Method for Estimation of Favipiravir API and It's Tablet Dosage Form Using Quality by Design Approach

Favipiravir is a pyrazine carboxamide derivative discovered by Toyama chemical of Japan to act against many RNA viruses (potent broad spectrum inhibitor of Influenza & other RNA viruses). The present study gives the development of the RP HPLC method by using Quality by Design followed by validation of developed method for estimation co Favipiravir in bulk and tablet dosage form. The central composite method was applied on organic Phase concentration & buffer PH for initial Screening studies. The selection of optimum chromatographic condition was carried out by design space numerical, graphical optimization on retention time, Peak asymmetry & Theoretical Plates. Optimized analytical method consisted Acetonitrile: water (80:20%v/v) as mobile phase, pH 5, flow rate 1ml/min, a wavelength 323 nm. Favipiravir was eluted with retention time 2.7 min & peak area 51248, Favipiravir showed good linear relationship in range of 10-90 microg/ml with a correlation coefficient of 0.9985. The % RSD for intraday, inter day precision & Repeatability was found to be 0.88, 0.65 & 0.97 respectively. Limit of detection & quantification was found to be 104.44 microg/ml &. 316.5 microg/ml. The method validation parameters were in the prescribed limit as per ICH guidelines. Copyright © 2022 Wolters Kluwer Medknow Publications. All rights reserved.